FW: RE: anti beta galactosidase immunos
Sent: Tue, 28 Jan 2003 10:11:55 -0500
Subject: RE: anti beta galactosidase immunos
An interesting development in search for bgal antibody that works in paraffin embedded tissue.
antibody from Developmental Studies Hybridoma Lab, 40-1a is working well and reliably in mouse embryos fixed in Dent's, with citrate solution boiling. Also able to use x-gal staining in Dent's fixed embryos, but not as intense as 4% para fixed.
I am still working on getting reliable bgal antibody results in para fixed, paraffin embedded. Will keep you posted. Thanks to Carl , Gayle, Donna, Hiro and so many others who have offered advice on antibodies and antigen retrieval. I may finish this thesis yet! Maureen @ East Carolina University.
From: "Martin, Ronald" <Ronald.Martin@umassmed.edu>
Sent: Fri, 03 Jan 2003 12:40:02 -0500
Subject: anti beta galactosidase immunos
Does anyone have a anti beta galactosidase immuno stain procedure for ffpe mouse tissue that you are willing to share? I have a PI who is looking for a procedure.
Ron Martin, HTL (ASCP)
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