FW: Elastic stain variations

From:"Smith, Allen"
 
 

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

-----Original Message-----
From: Smith, Allen
Sent: Saturday, January 25, 2003 8:39 AM
To: 'Aziz Ahmed Jafri'
Subject: RE: Elastic stain variations

So far as I know, elastin is the same in both places.
The difference is that fibrillin accompanies the elastin in the connective tissue but not in the arteries.
Thus one would like to have a stain for fibrillin.
All of the classical stains for elastic fibers stain the elastin not the fibrillin.
You need an antibody to fibrillin, but I don't remember ever having seen one for sale.
 

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

-----Original Message-----
From: Aziz Ahmed Jafri [mailto:aaj1@columbia.edu]
Sent: Friday, January 24, 2003 8:22 PM
To: Shaun Kay
Cc: histonet@pathology.swmed.edu
Subject: Re: Elastic stain variations

Try Orcein stain for elastic fibers.

Shaun Kay wrote:

Dear AllI have been asked whether it is possible to perform an elastic stain which differentiates elastic fibres in tissue compared to elastic fibres in blood vessels.Is there such a variation to our old EVG's which does this?regardsShaun Kay 
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