Cell Marque has a polyclonal equivalent to the DAKO product. When we found out about the DAKO backorder, we bought the Cell Marque antibody and now use it at 1:1000, steam in Citrate buffer for 15 min., with Powervision+ detection. It looks great!
The literature will suggest that the monoclonal CD3(clone PS1) is a very good antibody, perhaps a wee bit more specific than the polyclonal antibody... it has been my experience however that it must be used at a much higher dilution (around 1:40-1:50), making the polyclonal far more economical.
Robert L. Lott, HTL(ASCP)
Manager, Anatomic Pathology
Baptist Health System
800 Montclair Road
Birmingham, AL 35213
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