AW: cutting trouble....

From:Margarete Vermehren

We had a similar problem. It was crucial to breathe on the block before
cutting. In our case - ISH - breathing was forbidden: swabbing with a damp
(a.d.+DEPC) tissue did the trick.
Good luck,

Margarete Vermehren
LMU M#252#nchen / Tieranatomie II
Tel:  +49 89 21805857
Fax: +49 89 21802569

-----Urspr#252#ngliche Nachricht-----
Gesendet: Thursday, January 30, 2003 12:44 AM
Betreff: cutting trouble....

      I have a cutting problem with parrafin block... I dont know where is
the problem... When we cut the kidney, liver or pituitary and some other
tissues (mouse), the cut seem like dry and where the tissu is in the block,
the parraraffin roll.  Firstly, I tried to change the processing for to be
sure that it's not the deshydratation. The temperature of the parrafin is
about 60 C in the proccessor and the embedding station. Normally, we let the
block about 30min in the ice before cutting but now we need more than 1:30
hre for to have a nice section. I think to change my parraffin... I use
tissueprep 2 from fisher. What do you think? Help me!!!!! :)

Patrice Boulianne
 Histopathotechnologist RT

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