I know it is not commonly done, but we are looking to assess levels of tyrosine phosphorylation in frozen sections (and whole mounts) of retina via immunohistochemistry.
I'd be very keen to see any protocols of people who have undertaken immunohistochemical studies of tyrosine phosphorylation.
In particular if anyone knows of a tissue (or cell type) with a long of endogenous tyrosine kinase activity to act as positive control. I have seem some literature suggesting that glia may be suitable.
Also any protocols for myeloperoxidase IHC (as opposed to tissue MPO activity)
Dr David van Reyk,
Dept Health Sciences,University of Technology,Sydney,
PO Box 123, Broadway, NSW 2007, AUSTRALIA
Ph 61-2-9514-2221 FAX 61-2-9514-2228
UTS CRICOS Provider Code: 00099F
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