I know it is not commonly done,  but we are looking to assess levels of tyrosine phosphorylation in frozen sections (and whole mounts) of retina via immunohistochemistry.
I'd be very keen to see any protocols of people who have undertaken immunohistochemical studies of tyrosine phosphorylation.
In particular if anyone knows of a tissue (or cell type) with a long of endogenous tyrosine kinase activity to act as positive control. I have seem some literature suggesting that glia may be suitable.

Also any protocols for myeloperoxidase IHC (as opposed to tissue MPO activity)

Ta,

Dr David van Reyk,
Dept Health Sciences,University of Technology,Sydney, 
PO Box 123, Broadway, NSW 2007, AUSTRALIA
Ph 61-2-9514-2221 FAX 61-2-9514-2228 
email david.vanreyk@uts.edu.au
http://www.science.uts.edu.au/health







UTS CRICOS Provider Code:  00099F

DISCLAIMER
========================================================================
This email message and any accompanying attachments may contain
confidential information.  If you are not the intended recipient, do not
read, use, disseminate, distribute or copy this message or attachments.
If you have received this message in error, please notify the sender
immediately and delete this message. Any views expressed in this message
are those of the individual sender, except where the sender expressly,
and with authority, states them to be the views the University of
Technology Sydney. Before opening any attachments, please check them for
viruses and defects.
========================================================================