need help F4/80

From:Robert Schoonhoven

Mouse IHC folks:

Has anyone had any luck with the rat anti mouse F4/80 (Accurate Scientific)?
This one just doesn't want to work for me on formalin fixed paraffin sections.

In brief I've tried the following:

Pretreatment with trypsin, HEIR citrate, and proteinase

primary dilution's of 1:50, 100 & 500

If anyone has had any good results using this antibody please contact me.


Robert Schoonhoven
Laboratory of Molecular Carcinogenesis
University of North Carolina


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