need help F4/80
Mouse IHC folks:
Has anyone had any luck with the rat anti mouse F4/80 (Accurate Scientific)?
This one just doesn't want to work for me on formalin fixed paraffin sections.
In brief I've tried the following:
Pretreatment with trypsin, HEIR citrate, and proteinase
primary dilution's of 1:50, 100 & 500
If anyone has had any good results using this antibody please contact me.
Thanks,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis
University of North Carolina
919-966-6343
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