insitu hybridization techniques for frozen sections
I'd like to pick your collective brains again. This time I'm looking for
information regarding doing ISH (RNA probes) on frozen section.
Does anybody out there do them routinely, and if so, why? Does one still
need to use Proteinase K to make the nucleic acids more accessible to the
probe? What is your fixative of choice? What other modifications to
"standard" ISH protocols are made for frozen sections? Anything else
I've left out?
Thanks for your advice!
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
<< Previous Message | Next Message >>