insitu hybridization techniques for frozen sections

From:"Johnson, Teri"

Me again...

I'd like to pick your collective brains again.  This time I'm looking for 
information regarding doing ISH (RNA probes) on frozen section.  
Does anybody out there do them routinely, and if so, why?  Does one still 
need to use Proteinase K to make the nucleic acids more accessible to the 
probe?  What is your fixative of choice?  What other modifications to 
"standard" ISH protocols are made for frozen sections?  Anything else 
I've left out?

Thanks for your advice!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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