freezing fixed tissues

From:Garry Ashton

Dear all,
I am about to freeze down some tissues that have been fixed in 10%NBF.
Is taking the tissue through a sucrose gradient and freezing slowing in OCT the best way to achieve reasonably good morphology, only I remember doing this several years ago and the morphological preservation was quite poor.
Thanks in advance for any help.
Garry
 
 
 
 
PICR
UK

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