em question


Dear EM users,

I am a novice em user and have recently noticed mold growing on my cassettes stored in Cacodylate Buffer. Can anyone tell me what I have done wrong? The following is the recipe for the buffer I used.
21.4g sodium cacodylate in 1000ml dw (0.1M) pH adjusted to 7.4
55g sucrose
0.2gm Calcium Chloride stored in refrigerator.

Thanks for your help,
Sue Tyler HT (ASCP)
Oxford Laboratory
904 South Morris Street
Oxford, MD 21654

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