Technovit

From:"Judith A. Stasko"

Hi everyone,
I am currently working on a project using Technovit.  I have not used
this particular product before but am familiar with resins.  I initially
had trouble getting it to polymerize but think I have that rectified.  I
am having trouble when I am cutting.  It appears to cut just fine but
when I place it on the water bath, it blows up into a million pieces. 
So I wonder if there is something to put in the water bath.  I have
called the company and they suggested Histonet.  In the meantime, I am
just putting the sections right on to the glass slides.
Next, has anyone done a silver stain on the sections?  The product
doesn't come with info on this particular stain.  We anticipate doing
that along with immuno.  Didn't want to reinvent the wheel if someone
already has this perfected.

TIA
Judi Stasko 


Judi Stasko
Head Laboratory Resources
National Animal Disease Center
Ames, IA
PH. # 515-663-7445
jstasko@nadc.ars.usda.gov



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