Re: zamboni vs straight paraformaldehyde
Perhaps as John Kiernan said "it could all be histomythology". Very likely
there is no difference in staining result between 4% parafomaldehyde- and
Zamboni-fixation. However, in a practical way there is. As far as I know 4%
paraformaldehyde in PBS should be freshly made up. Weighing, handling and
dissolving (60C) the harmful PFA powder is quite a pain. In contrast, the
Zamboni-fixative can be made up in a large volume, stored at room
temperature and seems to work for ever!
In case somebody want to test 4% paraformaldehyde in PBS, here is the
formula of Zamboni:
Distilled water 800 ml
NaOH 4.3 gram
Paraformaldehyde 20 gram
NaH2PO4.H2O 18.8 gram
Saturated picric acid solution (double filtered) 150 ml
Dissolve the NaOH first, then add paraformaldehyde, and stir until
Add the sodium dihydrogen phosphate, and stir until completely dissolved.
Add the saturated picric acid solution.
Check and/or adjust pH to 7.3 with 2 N NaOH.
Bring volume to 1 liter with distilled water.
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam, The Netherlands
David Van Reyk wrote:
>Date: 8 Jan 2003 05:30:54 -0600
>From: David Van Reyk
>Subject: zamboni vs straight paraformaldehyde
>happy new year to all,
>[I think my firts attempt at posting this inquiry failed]
>we have been provided with an immunohistochemical protocol for forzen
>that requires Zamboni's fixative. In our lab we normally use 4%
>paraformaldehyde. The protocol mentions that zamboni's is used to keep the
>sections from falling off the slides. Is this the only reason Zamboni's
>be used over straight paraformaldehyde or are there other advantages.
>Dr David van Reyk,
>Dept Health Sciences,University of Technology,Sydney,
>PO Box 123, Broadway, NSW 2007, AUSTRALIA
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