Re: liver autofluorescence

From:Dale Osborne

	We've had recommended to us to first block the sections with 
sodium borohydride (1mg/ml PBS).  We tried it once but without much 
success.  Since we do few livers, we haven't put much effort into finding 
a solution to the autofluorescence problem.

Good luck,
Dale Osborne
Washington Univ. School of Medicine
St. Louis, MO
On Wed, 8 Jan 2003, Jaynes, Florinda wrote:

> I am having trouble decreasing green autofluorescence in perfused (4% PFA)
> and subsequently frozen liver sections.  I am trying to use Alexa 488 as a
> secondary marker for my primary and am having a tough time detecting it over
> background.  Any suggestions?  
> Thank you very much-
> Kris

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