Re: liver autofluorescence
We've had recommended to us to first block the sections with
sodium borohydride (1mg/ml PBS). We tried it once but without much
success. Since we do few livers, we haven't put much effort into finding
a solution to the autofluorescence problem.
Washington Univ. School of Medicine
St. Louis, MO
On Wed, 8 Jan 2003, Jaynes, Florinda wrote:
> I am having trouble decreasing green autofluorescence in perfused (4% PFA)
> and subsequently frozen liver sections. I am trying to use Alexa 488 as a
> secondary marker for my primary and am having a tough time detecting it over
> background. Any suggestions?
> Thank you very much-
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