Re: em question

From:Geoff McAuliffe

    I have had mold growing in older cacodylate buffer kept in the cold, but it takes awhile. The sucrose gives the mold something extra to eat. The only 'solution' I know of it to process the tissue
prompty. In the meantime, put the tissue in a clean cassett in fresh buffer. wrote:

> Dear EM users,
> I am a novice em user and have recently noticed mold growing on my cassettes stored in Cacodylate Buffer. Can anyone tell me what I have done wrong? The following is the recipe for the buffer I used.
> 21.4g sodium cacodylate in 1000ml dw (0.1M) pH adjusted to 7.4
> 55g sucrose
> 0.2gm Calcium Chloride stored in refrigerator.
> Thanks for your help,
> Sue Tyler HT (ASCP)
> Oxford Laboratory
> 904 South Morris Street
> Oxford, MD 21654
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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