Re: Mast cells.

From:Katri Tuomala

The controversy with CD68 as a macrophage marker is that it does not label
all macrophages. To my knowledge there is not one single antibody or stain
that will specifically mark all macrophages only.
Katri

Katri Tuomala
Dept. of Pathology
St.Joseph's Health Care
Hamilton, Ontario, Canada

----- Original Message -----
From: "Tony Henwood" 
To: "'Ian Montgomery'" ;

Sent: Tuesday, January 07, 2003 6:08 PM
Subject: RE: Mast cells.


> Ian,
>
> Possibly the following:
>
> Macrophages - stain with a CD68 antibody using IPX
> Fibroblasts - stain for Factor 13a using IPX
> Mast cells - stain with alcian blue or toluidine blue
>
> If my memory serves me correctly, there was some controversy re macrophage
> staining using CD68. Possibly a search would help.
>
> I would do the ipxs, using a double staining system, and then do the mast
> cell staining. Would there be a problem using antigen retrieval and then
the
> mast cell stain? Is alcian blue or toluidine blue colour fast following
> retrieval? I don't know.
>
> What do you think?
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at  Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: (02) 9845 3306
> Fax: (02) 9845 3318
>
> http://www.histosearch.com/homepages/TonyHenwood/default.html
> 
> http://us.geocities.com/tonyhenwoodau/index.html
> 
>
>
>
> -----Original Message-----
> From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk]
> Sent: Wednesday, 8 January 2003 3:31
> To: histonet@pathology.swmed.edu
> Subject: Mast cells.
>
>
>         Any suggestions for a stain or combination of stains, conventional
> or IHC, that will enable me to distinguish between mast cells, macrophages
> and fibroblasts.
> Ian.
>
>
> Dr. Ian Montgomery,
> Histotechnology,
> Graham Kerr Building,
> Institute of Biomedical & Life Sciences,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 339 8855
> Office: 4652
> Lab: 6644.
> Pager: 07625 702883
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
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