RE: need help F4/80

From:Cynthia Favara


Over the years I have tried with no success to get reliable staining on FFPE
mouse tissue with little success.

What exactly to you mean by boosting the detection system? Biotin tyramide
amplification or something else that I am not aware of?

Would love to use this antibody reliably.


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Chris van der Loos []
Sent: Friday, January 10, 2003 1:07 AM
Subject: RE: need help F4/80

Dear Robert,
Recently, I saw the same problem with the very same antibody with one of my 
colleges. Boosting the detection system has helped him quite a lot.

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam, The Netherlands

 >Date: 9 Jan 2003 06:31:01 -0600
 >From: Robert Schoonhoven 
 >Subject: need help F4/80
 >Mouse IHC folks:
 >Has anyone had any luck with the rat anti mouse F4/80 (Accurate
 >This one just doesn't want to work for me on formalin fixed paraffin 
 >In brief I've tried the following:
 >Pretreatment with trypsin, HEIR citrate, and proteinase
 >primary dilution's of 1:50, 100 & 500
 >If anyone has had any good results using this antibody please contact me.
 >Robert Schoonhoven
 >Laboratory of Molecular Carcinogenesis
 >University of North Carolina

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