RE: need help F4/80
Recently, I saw the same problem with the very same antibody with one of my
colleges. Boosting the detection system has helped him quite a lot.
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam, The Netherlands
>Date: 9 Jan 2003 06:31:01 -0600
>From: Robert Schoonhoven
>Subject: need help F4/80
>Mouse IHC folks:
>Has anyone had any luck with the rat anti mouse F4/80 (Accurate Scientific)?
>This one just doesn't want to work for me on formalin fixed paraffin
>In brief I've tried the following:
>Pretreatment with trypsin, HEIR citrate, and proteinase
>primary dilution's of 1:50, 100 & 500
>If anyone has had any good results using this antibody please contact me.
>Laboratory of Molecular Carcinogenesis
>University of North Carolina
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