Dear Robert,
Recently, I saw the same problem with the very same antibody with one of my 
colleges. Boosting the detection system has helped him quite a lot.

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam, The Netherlands

 >Date: 9 Jan 2003 06:31:01 -0600
 >From: Robert Schoonhoven 
 >Subject: need help F4/80
 >Mouse IHC folks:
 >Has anyone had any luck with the rat anti mouse F4/80 (Accurate Scientific)?
 >This one just doesn't want to work for me on formalin fixed paraffin 
sections.
 >In brief I've tried the following:
 >Pretreatment with trypsin, HEIR citrate, and proteinase
 >primary dilution's of 1:50, 100 & 500
 >If anyone has had any good results using this antibody please contact me.
 >Thanks,
 >Bob
 >Robert Schoonhoven
 >Laboratory of Molecular Carcinogenesis
 >University of North Carolina