RE: freezing fixed tissues

From:

Tony Henwood

I would do the sucrose gradient, but I would rapidly freeze, using liquid Nitrogen or similar. Slow freezing will give you large ice crystals, and therefore poor morphology

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Garry Ashton [mailto:GAshton@PICR.man.ac.uk]
Sent: Friday, 10 January 2003 23:12
To: 'histonet'
Subject: freezing fixed tissues

Dear all,

I am about to freeze down some tissues that have been fixed in 10%NBF.

Is taking the tissue through a sucrose gradient and freezing slowing in OCT the best way to achieve reasonably good morphology, only I remember doing this several years ago and the morphological preservation was quite poor.

Thanks in advance for any help.

Garry

PICR

UK

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