Patti & interested others:
For solution pcr use, we have an Eppendorf Mastercycler Gradient with a heat block that accompdates 1.0 mL, 0.5 mL or 96 well plates.
For in-situ pcr, we use a Lab-line Model 216 cycler (Fisher Scientific).
Reagents come from Promega or Perkin Elmer. We use Invitrogen for preparation of custom oligos and primers.
Hope that helps, Donna Montague
University of Arkansas for Medical Sciences
Departments of Physiology & Orthopaedic Surgery
4301 W. Markham St. # 505
Little Rock, AR 72205
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