RE: Mast cells.

From:

Tony Henwood

Ian,

Possibly the following:

Macrophages - stain with a CD68 antibody using IPX

Fibroblasts - stain for Factor 13a using IPX

Mast cells - stain with alcian blue or toluidine blue

If my memory serves me correctly, there was some controversy re macrophage staining using CD68. Possibly a search would help.

I would do the ipxs, using a double staining system, and then do the mast cell staining. Would there be a problem using antigen retrieval and then the mast cell stain? Is alcian blue or toluidine blue colour fast following retrieval? I don't know.

What do you think?

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk]
Sent: Wednesday, 8 January 2003 3:31
To: histonet@pathology.swmed.edu
Subject: Mast cells.

Any suggestions for a stain or combination of stains, conventional or IHC, that will enable me to distinguish between mast cells, macrophages and fibroblasts.
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk





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