RE: Mast cells.

From:Tony Henwood

Possibly the following:
Macrophages - stain with a CD68 antibody using IPX
Fibroblasts - stain for Factor 13a using IPX
Mast cells - stain with alcian blue or toluidine blue
If my memory serves me correctly, there was some controversy re macrophage staining using CD68. Possibly a search would help.
I would do the ipxs, using a double staining system, and then do the mast cell staining. Would there be a problem using antigen retrieval and then the mast cell stain? Is alcian blue or toluidine blue colour fast following retrieval? I don't know. 
What do you think?

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Ian Montgomery []
Sent: Wednesday, 8 January 2003 3:31
Subject: Mast cells.

        Any suggestions for a stain or combination of stains, conventional or IHC, that will enable me to distinguish between mast cells, macrophages and fibroblasts.

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883

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