RE: Immuno Question

From:"Drew Sally A."

We have used Zymed/s polyclonal calretinin, BioCare Medical's polyclonal TdT and their monoclonal LMP with success on the Benchmark, Nexes and ES.  For the calretinin & TdT we use a pressure cooker for heat retrieval, for the LMP we only need Protease 1 for 8 minutes.  Please feel free to contact us if you would like more specifics.

Sally Ann Drew-MT(ASCP)
Univ. of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave. VAH-DM223
Madison, WI 53792-2472

-----Original Message-----
From: []
Sent: Tuesday, January 14, 2003 5:42 PM
To: histonet
Subject: Immuno Question

We are having trouble with the following immunos on our Ventanna Benchmark , TDT,Calretian, EpstienBarr, can anyone let us know a good staining protocol we have tried many with purchased controls with minimal luck any help would be appreciated .

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