RE: Help with a fixative

From:Walzer Susan

!0% NBF is made from 37% formaldehyde (100 % formaldehyde is a gas) In the
histology lab we use 37% formaldehyde as if it were 100%. The most commonly
used (from AFIP) buffering method is:
          37% formaldehyde (formalin)......100mls
          distilled water..................900mls
          sodium phosphate momobasic.......4 gms
          sodium phosphate dibasic.........6.5 gms
           

-----Original Message-----
From: Charles Read [mailto:csu15397@mail.claytonstate.net]
Sent: Tuesday, January 07, 2003 5:59 PM
To: histonet@pathology.swmed.edu
Subject: Help with a fixative


Hi guys, can smbdy help me by telling me a good all purpose fixative?  Isn't
Formalin a 37% solution of formaldehyde?  And what is 10% Buffered formalin?
Does that mean its a 10% solution of 37% formaldehyde?  All help is very
appreciated!!!!!




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