RE: Daily Digest

From:Rainbow Ron

Hi histonetters,

What are people using now instead of alcian blue 8GX?

Thanks in advance

Ron Rainbow
Scientist in Charge
Department of Anatomical Pathology
Royal Hobart Hospital
GPO Box 1061L
Hobart
Tasmania 
Australia 7001
Phone: 0362228771
Fax: 0362228191
Email: ron.rainbow@dchs.tas.gov.au




-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Tuesday, 14 January 2003 4:56 PM
To: HistoNet Server
Subject: Daily Digest



----------------------------------------------------------------------

Date: 13 Jan 2003 07:15:24 -0600
From: "Morken, Tim" 
Subject: RE: Smallpox protocols

Allen wrote:
<>

Yes,it does, that is what we use for our research and recommend for tissue
fixation.

Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
MS-G32 
1600 Clifton Road
Atlanta, GA 30333

PH 404-639-3964
FAX 404-639-3043
email tim9@cdc.gov 

- -----Original Message-----
From: Smith, Allen [mailto:asmith@mail.barry.edu]
Sent: Sunday, January 12, 2003 11:39 AM
To: Melissa Jensen
Cc: histonet@pathology.swmed.edu
Subject: RE: HISTONETTERS PLEASE READ!!! RE: Smallpox protocols


If smallpox rears its unlovely head again, there will probably be a period
where old fossils who have been vaccinated (4 times in my case!) should do
all the handling of fresh tissues until the youngsters can be vaccinated.  
Does anyone know if formalin inactivates pox virus?  I'm sure the old
mercuric chloride fixatives do.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695 

- -----Original Message-----
From: Melissa Jensen [mailto:melzy@indytel.com]
Sent: Saturday, January 11, 2003 7:46 PM
To: histonet@pathology.swmed.edu
Subject: Re: HISTONETTERS PLEASE READ!!! RE: Smallpox protocols


Oh yes Vinnie.
- ----- Original Message -----
From: "Vinnie Della Speranza" 
To: ; ;

Sent: Friday, January 10, 2003 10:26 AM
Subject: HISTONETTERS PLEASE READ!!! RE: Smallpox protocols


> I hear you Tim but I have a concern and would appreciate your opinion
> about this. it is my understanding from what I've read that patients in
> the prodromal period, which can last 2-4 days and precedes appearance of
> a rash, can sometimes be infectious. we do forensic cases here. I can
> envision an individual who thinks he has the flu heading off to the
> local Eckerd's for over the coutner medication and is killed in a fatal
> car wreck. He ends up on my autopsy table as an MVA victim.
> I don't believe this scenario is outside the realm of possibilities.
>
> not wishing to cause anyone anxiety by bringing this up. I'm being told
> that mortuary and autopsy personnel are not among those to be
> vaccinated, at least initially, here. This may be based upon the belief
> that the Feds will assume responsibility in the event of a smallpox
> outbreak. I wonder if anyone has considered the scenario I've mentioned
>
>
>
>
>
> Vinnie Della Speranza
> Manager for Anatomic Pathology Services
> Medical University of South Carolina
> 165 Ashley Avenue  Suite 309
> Charleston, SC 29425
> Ph: 843-792-6353
> fax: 843-792-8974
>
> >>> "Morken, Tim"  01/10/03 08:31AM >>>
> Jude, and everyone else for that matter, PLEASE go to this website. It
> has a
> lot of info, including lab specimen collection. And PLEASE DO NOT EVEN
> THINK
> OF DOING FROZEN SECTIONS ON SMALLPOX!!! Frozens, or even an autopsy
> would
> have to be done in an air-isolatin room - which you most likely do not
> have.
> You would also need to wear isolation suits to do frozens. Even
> specimen
> collection is done in isolation with extreme precautions.
>
> On this website look for "Laboratory Testing", then go to Specimen
> Collection Guidelines, Guide-D, and also read the entire "Smallpox
> Response
> Plan & Guidelines"
>
> http://www.bt.cdc.gov/agent/smallpox/index.asp
>
>
> Tim Morken EMT(MSA), HTL(ASCP)
> Centers for Disease Control and Prevention
> Infectious Disease Pathology
> MS-G32
> 1600 Clifton Road
> Atlanta, GA 30333
>
> PH 404-639-3964
> FAX 404-639-3043
> email tim9@cdc.gov
>
>
>
>
> -----Original Message-----
> From: Carpenter, Judith A. [mailto:Jude.Carpenter@vtmednet.org]
> Sent: Friday, January 10, 2003 7:29 AM
> To: 'NSH'
> Subject: Smallpox protocols
>
>
> Does anyone have an established or proposed protocol for how
> they will deal with tissues from a "suspicious case" in which the
> clinician might suspect smallpox and who at their facility will be
> vaccinated up front ?
> Our facility is working on a Bioterrorism matrix determining who needs
> up
> front
> vaccinations should a smallpox threat present itself anywhere in
> the world including the USA (our Level one).  We will be given limited
> vaccine initially.
> The plan goes from Level one ("Convincing Threat" up to Level six where
> we
> have multiple
> cases at our facility.
> We must determine who would get the limited number of vaccinations at
> Level
> one
> (once we go beyond that the state will institute their plan).
> We have chosen autopsy personnel and a few individuals in the Surgical
> Dissection area
> who might do frozen sections on suspicious cases.
> Of course anytime a case is confirmed small pox we will cease to do
> frozens
> on any
> suspicious cases and will not autopsy deceased small pox victims.
> I was asked to throw this question out to you folks to see if anyone
> else
> has a protocol
> we may learn from.
> Appreciate your help and Happy Friday-
> Jude
> Jude Carpenter, BS, HTL(ASCP)
> Chief Technologist
> FAHC Histopathology
> 111 Colchester Ave.
> Burlington, VT  05401
> (802)847-5116
> FAX(802)847-3509
> jude.carpenter@vtmednet.org
>
>
>
>
>




----------------------------------------------------------------------

Date: 13 Jan 2003 07:45:31 -0600
From: antje.marcantonio@pharma.novartis.com
Subject: Re: need help F4/80


Bob,

we use the F4/80 antibody from Serotec for both frozen and formalin fixed 
paraffin sections.
For this antibody the fixation mustn't be longer than 2-3 days in 
formalin. This is true for an optimal staining for many antibodies, but 
with the F4/80 it is really obligatory.
I never tried any boosting here because there is always a little bit of 
background, which we reduced to a minimum by adding mouse serum to the 
blocking solution.
Pretreatment: 0.2% trypsin for 10 min
We use the primary 1:100 diluted in the blocking solution (10% normal 
rabbit serum plus 2.5 % mouse serum) , incubation overnight (for frozens 
60 min, rt).
As secondary: rabbit anti-rat, mouse adsorbed , Vector
I have to admit that staining of frozen sections is less problematic but 
we are happy with our staining on FFPE , too.
Good luck !

Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basle/Switzerland





Robert Schoonhoven 
09.01.2003 13:20

 
        To: 
        cc:     "'histonet@pathology.swmed.edu'"

        Subject:        need help F4/80


Mouse IHC folks:

Has anyone had any luck with the rat anti mouse F4/80 (Accurate 
Scientific)?
This one just doesn't want to work for me on formalin fixed paraffin 
sections.

In brief I've tried the following:

Pretreatment with trypsin, HEIR citrate, and proteinase

primary dilution's of 1:50, 100 & 500

If anyone has had any good results using this antibody please contact me.

Thanks,
Bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis
University of North Carolina

919-966-6343







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Bob,

we use the F4/80 antibody from Serotec for both frozen and formalin fixed paraffin sections.
For this antibody the fixation mustn't be longer than 2-3 days in formalin. This is true for an optimal staining for many antibodies, but with the F4/80 it is really obligatory.
I never tried any boosting here because there is always a little bit of background, which we reduced to a minimum by adding mouse serum to the blocking solution.
Pretreatment: 0.2% trypsin for 10 min
We use the primary 1:100 diluted in the blocking solution (10% normal rabbit serum plus 2.5 % mouse serum) , incubation overnight (for frozens 60 min, rt).
As secondary: rabbit anti-rat, mouse adsorbed , Vector
I have to admit that staining of frozen sections is less problematic but we are happy with our staining on FFPE , too.
Good luck !

Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basle/Switzerland




Robert Schoonhoven <rschoon@email.unc.edu>

09.01.2003 13:20

       
        To:        
        cc:        "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
        Subject:        need help F4/80



Mouse IHC folks:

Has anyone had any luck with the rat anti mouse F4/80 (Accurate Scientific)?
This one just doesn't want to work for me on formalin fixed paraffin sections.

In brief I've tried the following:

Pretreatment with trypsin, HEIR citrate, and proteinase

primary dilution's of 1:50, 100 & 500

If anyone has had any good results using this antibody please contact me.

Thanks,
Bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis
University of North Carolina

919-966-6343




- --Boundary_(ID_lil66YfzQo4Efsjzur1I8Q)-- ---------------------------------------------------------------------- Date: 13 Jan 2003 09:46:12 -0600 From: Nancy E Weber Subject: Re: Claw from dogs, Hello, What works pretty good, is to place the nail into a weigh boat and completely submerge it in Nair, the hair removal product you can usually get in any pharmaceutical section of any store. You should cover the weigh boat in plastic so the Nair doesn't dry out as fast. It takes a few days, but keep iit in the Nair until the toenail becomes pretty pliable, then process as usual. The Nair helps to break down the keratin. Nancy Nancy Weber Research Associate Veterinary Clinical Sciences Ohio State University ----- Original Message ----- From: Lab BioVet AB To: histonet@pathology.swmed.edu Sent: Friday, January 10, 2003 10:21 AM Subject: Claw from dogs, I am working in a Clinical Veterinary histo pathology lab. From time to time we get claw from dog And we have some problem to section this, we try decalcified the specimens but I don't works we also got e tip that cede wood oil could works, but no way. Is there someone, which have some useful tips for this kind of specimens? Britt-Marie Smedberg BioVet AB biovetlab@biovet.se ---------------------------------------------------------------------- Date: 13 Jan 2003 10:31:19 -0600 From: Robert Schoonhoven Subject: Thank You!!! for the help F4/80 Thanks to all of you for the help with the F4/80!!! It seems that the problem is with the primary anti-body. I placed an order with Serotec and I'll be giving that a try over the next few days. Again, many thanks to all of you (too many to thank individually) that came forth with the helpful suggestions (and procedures!). Robert Schoonhoven ---------------------------------------------------------------------- Date: 13 Jan 2003 11:00:36 -0600 From: "Bright, Alan" Subject: RE: new cryostat Attn: Evelyn Kaplan, Thank you for your enquiry. Please let me have your telephone number and a good time to contact you. Best regards Alan Bright Bright Instrument Co. Ltd. St. Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel: +44(0) 1480 454528/451980/451499 Fax:+44(0) 1480 456031 Email: ABright@Brightinstruments.com Web Site: BrightInstruments.com - -----Original Message----- From: Ms. Evelyn Kaplan [mailto:ekaplan@squ.edu.om] Sent: 13 January 2003 05:14 To: Histonet Subject: new cryostat Good morning, I am interested in knowing more about the new 7500 cryostat from Bright Instruments, which includes an automatic decontamination facility. So if Alan Bright is reading this, please could you contact me? Thanks. Would be delighted to hear from anyone who has used this instrument. Regards, Evelyn Kaplan Dept of Pathology, Sultan Qaboos University, Muscat, Oman ******************* NOTE ******************* There may be important message content contained in the following MIME Information. ******************************************** - ------------------ MIME Information follows ------------------ This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. - --Boundary_(ID_cwEXOay85MXtXaYbEvB6wA) Content-type: text/plain Content-transfer-encoding: 7BIT <<<<<< See above "Message Body" >>>>>> - --Boundary_(ID_cwEXOay85MXtXaYbEvB6wA) Content-type: text/html Content-transfer-encoding: 7BIT RE: new cryostat

Attn: Evelyn Kaplan,

Thank you for your enquiry.
Please let me have your telephone number and  a good time to contact you.


Best regards

Alan Bright

Bright Instrument Co. Ltd.
St. Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel: +44(0) 1480 454528/451980/451499
Fax:+44(0) 1480 456031
Email: ABright@Brightinstruments.com
Web Site: BrightInstruments.com


-----Original Message-----
From: Ms. Evelyn Kaplan [mailto:ekaplan@squ.edu.om]
Sent: 13 January 2003 05:14
To: Histonet
Subject: new cryostat

Good morning,

I am interested in knowing more about the new 7500 cryostat from Bright
Instruments, which includes an automatic decontamination facility. So if
Alan Bright is reading this, please could you contact me? Thanks. Would be
delighted to hear from anyone who has used this instrument.
Regards,
Evelyn Kaplan
Dept of Pathology,
Sultan Qaboos University,
Muscat, Oman

- --Boundary_(ID_cwEXOay85MXtXaYbEvB6wA)-- ---------------------------------------------------------------------- Date: 13 Jan 2003 11:00:58 -0600 From: BriggsK@drmc.drhsi.org Subject: Cryostat I am looking at the ThermoShandon Cryotome E cryostat and their semi-automated microtome. Would anyone be willing to share some feedback? Thanks, Kevin D. Briggs, MS, CT(ASCP) Team Leader/ Cytopathology/ Histopathology Services Danville Regional Medical Center 142 South Main Street Danville, VA 24541 Telephone: (434) 799-4470 ext.5451 Fax: (434) 799-2118 E-mail: briggsk@drhsi.org ---------------------------------------------------------------------- Date: 13 Jan 2003 14:45:48 -0600 From: "LaFriniere, Mike" Subject: Tennessee State Meeting VENDORS we still have a few spots left for you to exhibit..... The TSH has an exciting program going out this week in the mail.. Steven Slap...Microwave Fixation Robert Skinner...Decalcified Paraffin Preps Michael LaFriniere...Grossing Procedures Laura Mahood... Concepts in Cytopreparations Robert Skinner...Managing Muscular Specimens Robert Lott..HT(ASCP) Readiness The Tennessee State Meeting this year will be held at: The Edgewater Hotel 402 River Rd Gatlinburg, TN 37738 A select number of rooms are reserved for a special rate of $69.00 plus tax per night. For Hotel Reservations call: 1(800) 423-9582 Mention TSH Spring Meeting for special rate. If you are interested in exhibiting, contact: Michael LaFriniere NSH Region III Director, 423-495-6117 or by email ---------------------------------------------------------------------- Date: 13 Jan 2003 14:46:10 -0600 From: "LaFriniere, Mike" Subject: Sorry... forgot to put in the Tennessee Society meeting dates in Gatlinburg TN.. Thursday eve..March 6th- Saturday eve March 8th. Michael ---------------------------------------------------------------------- Date: 13 Jan 2003 15:30:19 -0600 From: Jackie.O'Connor@abbott.com Subject: Fixation of cytospins Greetings histonetters! What is the latest 'gold standard' for fixing cytospins destined for Immunocytochemistry? I'll be collecting HMVEC cells at passage 5 for ETA cytochemistry. Thanks in advance to all the brilliant minds I've come to admire so much!!! Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Fax 847.938.3266 ******************* NOTE ******************* There may be important message content contained in the following MIME Information. ******************************************** - ------------------ MIME Information follows ------------------ This is a multipart message in MIME format. - --Boundary_(ID_lVk1ukIKfpviCBl7Gob/6Q) Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7BIT <<<<<< See above "Message Body" >>>>>> - --Boundary_(ID_lVk1ukIKfpviCBl7Gob/6Q) Content-type: text/html; charset=us-ascii Content-transfer-encoding: 7BIT
Greetings histonetters!

What is the latest 'gold standard' for fixing cytospins destined for Immunocytochemistry?  I'll be collecting HMVEC cells at passage 5 for ETA cytochemistry.   Thanks in advance to all the brilliant minds I've come to admire so much!!!

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
847.938.4919
Fax 847.938.3266
- --Boundary_(ID_lVk1ukIKfpviCBl7Gob/6Q)-- ---------------------------------------------------------------------- Date: 13 Jan 2003 17:00:29 -0600 From: barbalb@itsa.ucsf.edu Subject: hematoxylin pH Hi all, Got a question from a pathologist (academic). He asked about the pH of hematoxylin. I assume it's acidic because of the acids we've all added to our home made heme batches, but I've never pH'd a batch of hematoxylin and find no reference to the pH in the few texts I've already consulted. What do you all have to say? Barbara Albert UCSF Medical Center San Francisco ---------------------------------------------------------------------- Date: 13 Jan 2003 17:00:52 -0600 From: Marilyn.S.Gamble@kp.org Subject: VIP-5 Owners I've been off the Histonet for an extended period of time, so forgive me if this subject is a repeat. We have had some problems with several VIP 5 processors, without error codes showing. To determine if we have an employee problem instead of an instrument problem, I'd like to check with other users of the new instrument. I (There are no problems with the older versions of the VIP). If you have had any issues with the instrument, would you send your responses to me please. Thank you. Marilyn Gamble Quality Assurance Coordinator, Cellular Pathology Regional Reference Laboratory, Sherman Way (818) 503-7029, tieline 397 E-mail: Marilyn.S.Gamble@kp.org ---------------------------------------------------------------------- Date: 13 Jan 2003 17:45:14 -0600 From: "Michelle D. Moore" Subject: Billing for technical fees Hello from chillsville I am in need of some last minute help, I was called by one of my clients and was told that all technical fees on specimens had to be charged by the collecting facility not the facility performing the testing. She stated to me that it was a government rule that took effect Jan 1, 2003 and told my to see if ASCP could help me. I have contacted ASCP via-email and have yet to hear back from them. I know there has to be something I can look up so that I know I'm doing the right thing but I'm having a very hard time finding the information I need. I have tried to look it up on the ASCP web site and have even tried to get my business office personnel to help me out, but nothing I have tried has given me what I'm looking for. HELP pleeeese!! If some wonderful person out there could help me out in finding the information I'm looking for I would be eternally grateful. I appreciate all your time and help with this problem and thank you in advance for any information. Have a great today and a better tomorrow. Michelle D. Moore HT(ASCP) tmhpath@amigo.net Histology Supervisor TMH Craig, CO ---------------------------------------------------------------------- Date: 13 Jan 2003 18:01:15 -0600 From: Cynthia Favara Subject: RE: VIP-5 Owners Marilyn, I have had the VIP since Oct 02 . Have no processing problems but I have three reagent bottles that I cannot remove. Sakura is sending a tech to fix the problem. Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 - -----Original Message----- From: Gamble,Marilyn S [mailto:Marilyn.S.Gamble@kp.org] Sent: Monday, January 13, 2003 3:44 PM To: 'Histonet' Subject: VIP-5 Owners I've been off the Histonet for an extended period of time, so forgive me if this subject is a repeat. We have had some problems with several VIP 5 processors, without error codes showing. To determine if we have an employee problem instead of an instrument problem, I'd like to check with other users of the new instrument. I (There are no problems with the older versions of the VIP). If you have had any issues with the instrument, would you send your responses to me please. Thank you. Marilyn Gamble Quality Assurance Coordinator, Cellular Pathology Regional Reference Laboratory, Sherman Way (818) 503-7029, tieline 397 E-mail: Marilyn.S.Gamble@kp.org ---------------------------------------------------------------------- Date: 13 Jan 2003 18:01:45 -0600 From: "Weems, Joyce" Subject: Billing for technical charges My recent answer to this question from one of our reference labs. CLIENT SERVICE BULLETIN HIGH PRIORITY December 20, 2002 The Centers for Medicare & Medicaid Services (CMS) issued a Program Memorandum (AB-02-177) Friday which appears to extend the BIPA-2000 Section 542 "grandfather" exception to December 31, 2003. The exception applies to anatomic pathology technical component services to hospital inpatients and outpatients by independent and other "outside" laboratories, when the referring hospital meets the Section 542 criteria. CMS achieved the extension by using its emergency administrative authority to change the "discard date" of the original Program Memorandum (AB-01-047) to December 31, 2003. It did not, however, change the body of the original text, which refers to the "2-year period beginning on January 1, 2001," thereby rendering the extension instruction to Medicare Part B carriers somewhat ambiguous. CMS has nonetheless assured the College of American Pathologists and others that the extension of the "grandfather" exception to December 31, 2003 is "for real." You can view or download Program Memorandum AB-02-177 by visiting website http://cms.hhs.gov/manuals/pm_trans/AB02177.pdf . I will alert you immediately should I find out anything that suggests we cannot take CMS at its word that the "grandfather" exception has indeed been extended beyond this year-end. Otherwise, you can "breathe easy" and continue to bill Medicare Part B in 2003 for the technical component of anatomic pathology services for inpatients and outpatients of qualifying hospitals served by your laboratory. Thank you, Dennis L. Padget, CPA, FHFMA Padget & Associates ******************* NOTE ******************* There may be important message content contained in the following MIME Information. ******************************************** - ------------------ MIME Information follows ------------------ This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. - --Boundary_(ID_gPbl+qcUQvY5Lx61fpVY4Q) Content-type: text/plain Content-transfer-encoding: 7BIT <<<<<< See above "Message Body" >>>>>> - --Boundary_(ID_gPbl+qcUQvY5Lx61fpVY4Q) Content-type: text/html Content-transfer-encoding: 7BIT Message

My recent answer to this question from one of our reference labs.

 

 

 

 

CLIENT SERVICE BULLETIN

HIGH PRIORITY

 

December 20, 2002

 

The Centers for Medicare & Medicaid Services (CMS) issued a Program Memorandum (AB-02-177) Friday which appears to extend the BIPA-2000 Section 542 "grandfather" exception to December 31, 2003. The exception applies to anatomic pathology technical component services to hospital inpatients and outpatients by independent and other "outside" laboratories, when the referring hospital meets the Section 542 criteria.

 

CMS achieved the extension by using its emergency administrative authority to change the "discard date" of the original Program Memorandum (AB-01-047) to December 31, 2003. It did not, however, change the body of the original text, which refers to the "2-year period beginning on January 1, 2001," thereby rendering the extension instruction to Medicare Part B carriers somewhat ambiguous. CMS has nonetheless assured the College of American Pathologists and others that the extension of the "grandfather" exception to December 31, 2003 is "for real." You can view or download Program Memorandum AB-02-177 by visiting website http://cms.hhs.gov/ma nuals/pm_trans/AB02177.pdf.

 

I will alert you immediately should I find out anything that suggests we cannot take CMS at its word that the "grandfather" exception has indeed been extended beyond this year-end. Otherwise, you can "breathe easy" and continue to bill Medicare Part B in 2003 for the technical component of anatomic pathology services for inpatients and outpatients of qualifying hospitals served by your laboratory.

 

Thank you,

Dennis L. Padget, CPA, FHFMA

Padget & Associates

 

- --Boundary_(ID_gPbl+qcUQvY5Lx61fpVY4Q)-- ---------------------------------------------------------------------- Date: 13 Jan 2003 19:00:34 -0600 From: Richard Cartun Subject: CD117 Has anyone ever received a request for CD117 immunostaining on an islet cell tumor (I assume for potential immunotherapy). R. Cartun ---------------------------------------------------------------------- Date: 13 Jan 2003 20:45:57 -0600 From: Bill Subject: Re: Billing for technical charges Re: Billing for technical charges
At 6:47 PM -0500 1/13/03, Weems, Joyce wrote:
I will alert you immediately should I find out anything that suggests we cannot take CMS at its word that the "grandfather" exception has indeed been extended beyond this year-end.

I have absolutely no idea what this explanation means. It reads like the gross description of a rare neurological degenerative disease. Could someone translate it into English for a poor pathologist.

TIA,

Bill

-- 
_____________________________
Bill Blank
http://kernunnos.com (Celtic studies and numismatics)
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