Research Diagnostics Inc  offers a wide line of  antibodies. Since no one
antibody works best for all applications (flow cytometry, neutralization,
blotting, histochemistry, ELISA, etc), we offer many different types of
antibodies to help solve this problem. Please inquire for other applications
or types of antibodies not listed below. All products are for in vitro
research use only-not for use in or on humans or animals-not for use in
diagnostics. Price/availability/specifications subject to change without
notice. 


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CD146 (S-ENDO/MUC 18) Antibodies  



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Mouse anti-human CD146  antibodies 
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CATALOG#                 CLONE#                  Wokshop    Host
Form               Price 
RDI-CD146-SENDO      S-ENDO1                    VI           mIgG1
200Tests          $475.00 

RDI-CD146-SFT                "                               VI
mIgG1        FITC 100T       $475.00 

RDI-CD146-4BT            Com 7A4                       --          mIgG1k
Biotin              $475.00 

-a product of Biocytex Biotechnology 


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DATA SHEET: mouse anti-human CD146 (S-ENDO1)

Catalog#: RDI-CD146-SENDO      $475.00/vial  200 tests in 2ml 
Also available  FITC  labeled: cat#RDI-CD146-SFT     $475.00/100T in 1ml



Species: mouse IgG1

CLONE: S-Endo1 (also designated F4-35H7)

Immunogen:Human umbilical cord endothelial cells (HUVEC) 

Fusion Partner:X63Ag8/653 

Package: in PBS with 0.1% BSA and <0.1% sodium azide

Activity: Reacts with human CD146 , tested at VIth Workshop (code E057,
clone name F4-35117). Postive reactivity with Muc18 transfectants (Flow
cytometry, MUC 18 FC Protein (Elisa), Endothelial cells (HUVECS, HDMEC,
Eahy, SKMEL28 &  SBC12.  Group I (more than 300K sites per HUVEC). Mapped at
domain 3.


-using an indirect immunoperoxidase assay performed in microtiter plates,
S-ENDO-1 binds to cultured HUVEC but not to platelets 

-using an immunoperoxidase staining on frozen tissue sections, S-ENDO1
demonstrates in all tissues tested a strong reactivity to vascular
endothelium 

-Analysed by an indirect immunostaining method on bone marrow cells, the
S-ENDO1 related antigen was absent 

-Analyzed on blood cells, using flow cytometry, S-ENDO1 does not bind to
leucocytes, nor red cells or platelets 


Application: -Indirect immunofluorescence cell surface staining ,  ELISA and
histochemistry (purified format, frozen sections only-not paraffin) Titer
for each application 

-detection of endothelial cells in whole blood 


References: 

1)  Leucocyte Typing VI, Garland Press, NY , 1997 p755-759 

2) J. Immunol Methods 139(1991) 65-75 

3) Thromosis and Haemostasis 67 (1992) 147-153 



Storage: Store at 4 DEG C.. 


Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. It is the responsibility of the user to comply
with all local/state and Federal rules in the use of this product. We are
not responsible forany patent infringements that might result with the use
of or derivation of this product.



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Mouse anti-human CD146:Biotin conjugated   
cat#RDI-CD146-4BT   $475.00/100 tests 

Clone: Com7A4 

Isotype: mIgG1kappa 

Partner of fusion: X63Ag8/653 

Immunogen: Cd146 immunopurified molecule 


Specificity: This antibody is positive on CD146 transfectants. Thsi antibody
has been tested for CD146 capture in an Elisa using biotinulated F(ab')2
F4-35H7 for detection 


Applications: flow cytometry & Elisa 

Size  100 tests (10ul/test) 

Presentation: in PBS with 0.1% BSA, pH 7.4 and 0.1% sodium azide 


Storage: Stable for several weeks at 4 DEG C as undiluted form. For longer
storage, freeze undiluted product in samll aliquots at -20 DEG C or below.
Avoid repeated freezing and thawing. 



-All products are for in vitro research use only-Not for use in or on humans
or animals-Not for Diagnostic use. Not responsible for any patent
infringements with the use or derivation of these products.
Price/specifications/availability subject to change without notice. 

also available: non labeled   cat#RDI-CD146-4         $450.00/200tests 

                     FITC labeled   cat#RDI-CD146-4FT     $450.00/100 tests 



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Research Diagnostics Inc 
Pleasant Hill Road 

Flanders NJ 07836 

phone (800) 631-9384 or 

           (973) 584-7093 

fax      (973) 584-0210 

EMAIL:ResearchD@aol.com 



-----Original Message-----
From: Turriago, Adriana [mailto:ATURRIAGO@chromavision.com]
Sent: Thursday, January 16, 2003 7:43 PM
To: histonet@pathology.swmed.edu
Subject: CD146


I attended an angiogenesis workshop at the NSH meeting in Long Beach, CA and
someone mentioned staining with CD146 (p1H12).  Has anybody have experience
with this antibody, have a vendor, or any additional information?

Any help you can give us will be greatly appreciated

Thanks,

Adriana

Adriana Turriago, MT (ASCP)
Laboratory Manager
Assay Development
Chromavision Medical Systems
(949) 443-3373 Ext. 279
aturriago@chromavision.com