RE: Bacteria Stain

From:"Johnston, Kathy"

We currently are doing the Brown and Brenn, but believe me, if there is a
bug stain that is not easy, it is this one.  I am currently in the works (or
hoping anyway!) to switch over to the Brown and Hopps for everyday use.  We
are currently using this method for on call kits, for the reason that it is
more goof proof, and techs that might do 1 gram a year on-call will be using
this kit.

I vote for the Brown and Hopps!

Kathy Johnston
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093

-----Original Message-----
From: Dunn-Jena, Patsy A []
Sent: Thursday, January 16, 2003 1:31 PM
Subject: Bacteria Stain

Need to know which stain most of you feel most comfortable with to check for
bacteria.  Was instructed to do a Brown & Brenn which has not come out to my
satisfaction any of the 3 times I have done it.  FINALLY have them convinced
to let me try a Brown & Hopps.  Any words of advice would be greatly
appreciated.  This is being done on Formalin Fixed, EDTA decalcified
paraffin embedded bone/tooth sections. 

Patsy Dunn-Jena, RVT, LAT, HT (ASCP)
Indiana University School of Dentistry
Mineralized Tissue and Histology Research Laboratory
Indianapolis, IN

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