RE: Bacteria Stain

From:Tony Henwood

RE: Bacteria Stain

I would use a giemsa stain for a few minutes for a rapid assessment. We use a Gram-Tworts for routine gram staining.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Dunn-Jena, Patsy A []
Sent: Friday, 17 January 2003 7:31
Subject: Bacteria Stain

Need to know which stain most of you feel most comfortable with to check for bacteria.  Was instructed to do a Brown & Brenn which has not come out to my satisfaction any of the 3 times I have done it.  FINALLY have them convinced to let me try a Brown & Hopps.  Any words of advice would be greatly appreciated.  This is being done on Formalin Fixed, EDTA decalcified paraffin embedded bone/tooth sections.

Patsy Dunn-Jena, RVT, LAT, HT (ASCP)
Indiana University School of Dentistry
Mineralized Tissue and Histology Research Laboratory
Indianapolis, IN

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