Dear HistoNet,
    I usually do Affymetrix GeneChip and spotted array work, but I have an
investigator with a question that I could not answer, so I thought I would
try everyone here.  This investigator would like to induce a defect in the
tibia of rabbits, wait a specific time to allow for healing, and then
section the bone for use on a Laser Capture Microscope.  The goal would be
to isolate the RNA of the different cell populations present during the time
line and then use that RNA on Afftmetrix GeneChips to check for differences
in expression.
    I tried doing some background research and found a protocol from DeCal
using their ImmunoCal solution to decalcify the bone and they say that this
will preserve RNA.  Does anyone know if this will work?
    My other question is how to best stain the bone and cartilage once it
has been sectioned?  I just need enough stain to be able to differentiate
between the different cells without killing the RNA.  Any help anyone might
be able to lend would be GREAYLY appreciated!!  I have only ever sectioned
organs and tumor tissue, so I'm pretty much helpless.

Thank you!!!
Lora Huang
Univ of Iowa DNA Facility
----- Original Message -----
From: "HistoNet Server" 
To: "Lora Huang" 
Sent: Thursday, January 16, 2003 9:59 AM
Subject: re: subscribe


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