Need to know which stain most of you feel most comfortable with to check for bacteria.  Was instructed to do a Brown & Brenn which has not come out to my satisfaction any of the 3 times I have done it.  FINALLY have them convinced to let me try a Brown & Hopps.  Any words of advice would be greatly appreciated.  This is being done on Formalin Fixed, EDTA decalcified paraffin embedded bone/tooth sections. 

Patsy Dunn-Jena, RVT, LAT, HT (ASCP)
Indiana University School of Dentistry
Mineralized Tissue and Histology Research Laboratory
Indianapolis, IN
padunnje@iupui.edu