|From:||"Dr. Ian Montgomery" |
there has been series of postings regarding overprocessed tissue that
have left me totally confused. The object in processing and embedding in
paraffin wax is the complete removal of water from tissue and it's
replacement by wax. Therefore if overprocessing has occurred tissue
has been lost and replaced by wax. If that is what's meant then the
overprocessed tissue is only fit for the rubbish bin. I really think that
the term overprocessing should be dropped and replaced by
something more suitable and probably more accurate such as under
processed, mis-processed, it wasn't me it was the machine and so
forth. From the descriptions people give I think water is still present
in the tissue or users have moved away from "proper" clearing
agents and rely on xylene or a substitute as the agent of choice which
can result in "crumbly" blocks. I still use chloroform,
cedarwood oil etc., "real" clearing agents and when the tissue
has been thoroughly dehydrated, cleared and embedded the resulting blocks
are a dream.
have the next wee problem. "How long did you fix the tissue."
"Oh, not very long, it's only been in Bouin for a couple of months.
That's alright, isn't it."
Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 332 8855 Extn.6644.
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