Some people have asked to see my processing schedule  to help determine if 
in fact our mouse tissue is being over processed.  After fixing for 48hrs 
and then being held in 70% ethanol until processing(could be a few 
weeks)  mouse tissues (about 3mm in size ) are processed by the following 
schedule:

70% ethanol              15min             Ambient 
temperature          Vacuum     *all solution are also
80% ethanol              15 min            Ambient 
temperature          Vacuum       stirring.
95% ethanol              20 min            Ambient temperature          Vacuum
95% ethanol              30 min            Ambient temperature          Vacuum
absolute ethanol        20 min            Ambient temperature          Vacuum
absolute ethanol        30 min            Ambient temperature          Vacuum
Xylene                      30 min            Ambient 
temperature          Vacuum
Xylene                      30min             Ambient 
temperature          Vacuum
Paraplast                 45 min            Ambient 
temperature          Vacuum
Paraplast                 45min             Ambient 
temperature          Vacuum
Paraplast                 45min             Ambient 
temperature          Vacuum


Thanks,
Renee


Renee Hoyle-Thacker
CIIT Centers for Health Research
6 Davis Dr.
Research Triangle Park NC 27709
Voice: (919) 558-1322