I have some labs who bring me hunks of frozen skeletal muscle for frozen 
sectioning. They clamp the muscle and snap freeze it and then store it in a 
microcentrifuge tube at minus 80 until needed. When I get the muscle I have 
to attach it to the cryostat chuck with OCT without thawing any of the tissue.
What I have had to do is make a base of OCT and just as it is about to 
freeze over I stick only a tip of the tissue into it. However when 
sectioning I'm cutting only the tissue with no supporting medium around it 
and this ain't easy!
If any other labs are doing this and would want to share how they are going 
about it I'd be really grateful!!!
Thanks!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html