decalcified tissues and processing

From:Linda Jenkins

Debbie,
	This is a common problem with decals.  Modification of the processing 
protocol is necessary to keep specimens from becoming almost as hard as 
before decalcification.  Gayle Callis and Diane Sterchi have done many 
workshops on this subject and have just recently edited a processing manual 
for the VIR committee ( Gayle, is this available for purchase, yet?).  I 
think there may be one or two processing protocols in there that may help.
	If all else fails, bounce it off the wall...just kidding...although I've 
been severely tempted by some of the decals I've had to cut.
	Linda

Linda Jenkins, HT
Bioengineering
Clemson University
864.656.5553





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