cytospin storage

From:Mitramf@aol.com

Hi everyone. We have some questions in our lab regarding the storage of 
cytospin slides (of bone marrow monocytes). We air dry our slides for 24 hrs, 
then store them at -80 degrees in slide boxes. We have not been using 
dessacents, nor have we fixed the slides before storage. Could either of 
these procedures pose serious problems? If so, is there any way of correcting 
them? Recently, as we have started retrieving our slides for staining and 
analysis, the pathologists have informed us that they are not happy with the 
morphology of our cells. 
Thanks,
Mitra
University of Minnesota




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