For clarification: I am being asked to paraffin process a fixed unprocessed 
section that is already 300 microns thick for 5-10 micron paraffin 
sections.

Bert

-----Original Message-----
From:	Bert Dotson [SMTP:amdj@duke.edu]
Sent:	Wednesday, January 23, 2002 3:21 PM
To:	Histonet (E-mail)
Subject:	Paraffin processing brain slices

A pharmacology researcher here assures me that a number of published 
studies indicate paraffin processing of 300 micron thick (thin if you ask 
me) slices of rat or mouse brain. Of course the procedure reported is 
"slices are paraffin processed and sectioned." Has anyone out there done 
this? Any particular tips or tricks? I am assuming a support medium like 
agar is used prior to processing.

Thanx in advance
Bert Dotson