Re: fixation of breast specimens for prognostic markers

From:

Amos Brooks

fixation of breast specimens for prognostic markers

Hi,

For optimal processing times I would recommend that you refer to the packaging insert to the ER, PR and H2N staining kits that you are using. These have the recommended fixation times. Actually the H2N ISH kit from Vysis has recommended tissue sizes for processing too. I think most recommend 24-48 hours for fixation.

I kind of like this. For years techs have been trying to convince pathologists, and more commonly residents, that they cannot stuff the whole breast into a single cassette for 10 min then put it in a routine processor. Now there is a test that will be adversely affected if they do this.

Amos Brooks

----- Original Message -----

From: Van Eyck, Deb

To: 'histonet@pathology.swmed.edu'

Sent: Friday, January 25, 2002 5:52 PM

Subject: fixation of breast specimens for prognostic markers

Hello all,
I would like to get a sample of responses from people about what they think is the optimal time and procedure for fixing breast specimens for doing immuno for ER, PR and Her 2 Neu. To include core samples, and lumpectomies with types of fixatives and times. Especially those using an streptavidin - biotin detection system. What about weekend specimens? Thanks- all input is much appreciated---I will share the responses.

Deb Van Eyck, Pathology Team Leader
Waukesha Memorial Hospital
Waukesha, WI 53188
262 928-2112
deb.vaneyck@phci.org

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