|From:||Barry Rittman |
2. While we used the above extensively in the mediaeval days of histology, for better adhesion, used to smear the glycerin albumen on slide, heat the slide in a bunsen flame until fumes given off. this produces better adhesion that glycerin albumen alone. Be careful the fumes ar toxic, best done in a fume hood.
3. Would recommend that you keep the nail on the slide
using a covering of celloidin. Flood slide with 0.5% -1% celloidin in alcohol:ether
(after absolute ethanol), drain, allow to get almost dry then immediately
into 70% ethanol. Following staining you must not use absolute ethanol
in the dehydration but a mixture of ethanol:chloroform - (at least 15%
chloroform) for the final dehydration to prevent celloidin from dissolving.
Hope that this helps.
Please help me with the following two questions that my teacher posed to us:
1. How to prepare egg albumin. An end product that will smear on to the glass slide for tissue adhesion.
2. How to successfully keep nail samples adhere to the glass slide during staining. Are there alternative methods other than the product above?
I would greatly appreciate the help with these questions.