Re: egg albumin/nail adhesion to glass slides

From:Barry Rittman

1.    the process that we used many years ago was to use equal parts of egg white and glycerin. The egg white needed to be filtered using vacuum. Glycerin then added, then crystals of thymol to inhibit growth of molds. Would suggest chicken rather than emu eggs!
Making this is a rweal pain, and this is probably still available from supply houses - I would suggest getting supply from there rather than making it yourself.

2.    While we used the above extensively in the mediaeval days of histology, for better adhesion, used to smear the glycerin albumen on slide, heat the slide in a bunsen flame until fumes given off. this produces better adhesion that glycerin albumen alone. Be careful the fumes ar toxic, best done in a fume hood.

3.    Would recommend that you keep the nail on the slide using a covering of celloidin. Flood slide with 0.5% -1% celloidin in alcohol:ether (after absolute ethanol), drain, allow to get almost dry then immediately into 70% ethanol.  Following staining you must not use absolute ethanol in the dehydration but a mixture of ethanol:chloroform - (at least 15% chloroform) for the final dehydration to prevent celloidin from dissolving.
Hope that this helps.
Barry wrote:

Please help me with the following two questions that my teacher posed to us:

1.  How to prepare egg albumin. An end product that will smear on to the glass slide for tissue adhesion.

2.  How to successfully keep nail samples adhere to the glass slide during staining. Are there alternative methods other than the product above?

I would greatly appreciate the help with these questions.

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