Re: Use of outdated antibodies
This is from the CAP checklist for IHC:
ANP.22450 Phase II
Are storage conditions of immunoreagents optimal for maintaining expected
The storage conditions of the immunoreagents in use must be improved to
maintain their expected shelf life.
REFERENCES: 1) Tubbs RR, et al. Extension of useful reagent shelf life
beyond manufacturers' recommendations. Arch Pathol Lab Med.
1998;122:1051-1052; 2) Balaton AJ. Satisfactory performance of primary
antibodies beyond manufacturer's recommended expiration dates. Appl
Immunohisto Mol Pathol. 1999;7:221-225.
So, if I remember the articles correctly: yes, antibodies could to be used
beyond the manufacturer's recommended expiration dates. But you have to test
document that the antibodies are still good. On a routine basis.
Hope these were what you were looking for.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Smith, Sherry A. (Histology)"
Sent: Thursday, January 31, 2002 8:43 AM
Subject: Use of outdated antibodies
> Dear Histotechs.,
> Are there any known articles about the use of outdated antibodies?? I
> remember reading one article but can't seem to locate it now. Any info.
> would be greatly appreciated.
> Sherry Smith
> Presbyterian Hospital
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