Re: Use of outdated antibodies

From:Lee & Peggy Wenk

This is from the CAP checklist for IHC:

ANP.22450 Phase II

Are storage conditions of immunoreagents optimal for maintaining expected
shelf life?


The storage conditions of the immunoreagents in use must be improved to
maintain their expected shelf life.

REFERENCES: 1) Tubbs RR, et al. Extension of useful reagent shelf life
beyond manufacturers' recommendations. Arch Pathol Lab Med.
1998;122:1051-1052; 2) Balaton AJ. Satisfactory performance of primary
antibodies beyond manufacturer's recommended expiration dates. Appl
Immunohisto Mol Pathol. 1999;7:221-225.

So, if I remember the articles correctly: yes, antibodies could to be used
beyond the manufacturer's recommended expiration dates. But you have to test
 document that the antibodies are still good. On a routine basis.

Hope these were what you were looking for.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Smith, Sherry A. (Histology)" 
Sent: Thursday, January 31, 2002 8:43 AM
Subject: Use of outdated antibodies

> Dear Histotechs.,
> Are there any known articles about the use of outdated antibodies??  I
> remember reading one article but can't seem to locate it now.  Any info.
> would be greatly appreciated.
> Thanks,
> Sherry Smith
> Presbyterian Hospital
> This message and any included attachments are from NOVANT HEALTH INC.
> and are intended only for the addressee(s). The information contained
> herein may include trade secrets or privileged or otherwise confidential
> information. Unauthorized review, forwarding, printing, copying,
> distributing, or using such information is strictly prohibited
> and may be unlawful. If you received this message in error, or have
> reason to believe you are not authorized to receive it, please promptly
> delete this message and notify the sender by e-mail.
> Thank you.

<< Previous Message | Next Message >>