Re: Use of outdated antibodies

From:Lee & Peggy Wenk

This is from the CAP checklist for IHC:

ANP.22450 Phase II

Are storage conditions of immunoreagents optimal for maintaining expected
shelf life?

COMMENTARY:

The storage conditions of the immunoreagents in use must be improved to
maintain their expected shelf life.

REFERENCES: 1) Tubbs RR, et al. Extension of useful reagent shelf life
beyond manufacturers' recommendations. Arch Pathol Lab Med.
1998;122:1051-1052; 2) Balaton AJ. Satisfactory performance of primary
antibodies beyond manufacturer's recommended expiration dates. Appl
Immunohisto Mol Pathol. 1999;7:221-225.



So, if I remember the articles correctly: yes, antibodies could to be used
beyond the manufacturer's recommended expiration dates. But you have to test
and
 document that the antibodies are still good. On a routine basis.

Hope these were what you were looking for.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073


----- Original Message -----
From: "Smith, Sherry A. (Histology)" 
To: 
Sent: Thursday, January 31, 2002 8:43 AM
Subject: Use of outdated antibodies


> Dear Histotechs.,
>
> Are there any known articles about the use of outdated antibodies??  I
> remember reading one article but can't seem to locate it now.  Any info.
> would be greatly appreciated.
>
> Thanks,
> Sherry Smith
> Presbyterian Hospital
>
>
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