Sounds like a good application for Fred's method for using the fluorescent
property of eosin to mark blood vessels.  He wrote about here on histonet a
few weeks ago.
Patsy Ruegg

"Peter Cattin (PhD)" wrote:

> Hi all,
>
> I am looking to stain the blood vessels and red blood cells of a two day
> old zebrafish embryo.  The embryos are mounted in JB4 plastic resin and
> sectioned at 5 microns.  Most recipes are strong on alcohol which tends
> to blister the sections.  Does anyone have any suggestions?
>
> --
> Peter Cattin PhD
> Division of Molecular Medicine
> Faculty of Medical and Health Sciences
> University of Auckland
> Park Road, Grafton, Auckland.
> Private bag 92019, Auckland,
> New Zealand.
>
> Tel: +64-9-373-7599 xtn. 6373
> Fax: +64-9-373-7492