Re: Rapid stain for pneumocystis carinii
Tony Henwood in Australia recommends and describes a toluidine blue sulfation
technique for rapid demonstration of Pneumocystis carinii.
This technique was rather widely recommended in the mid 1980's at the height
of the AIDS epidemic - it was recommended in the standard Scott and Bailey
microbiology textbook for instance - and then it sort of disappeared. Do many
people have any actual experience of it - I've never seen it. I thought it
was displaced by rapid GMS microwave techniques.
With concentrated sulfuric acid and acetone, it's a somewhat hazardous
technique, particularly when performed by an inexperienced worker in the
middle of the night ... and as for turning pathologists loose on it solo ...
<< Previous Message | Next Message >>