Tony Henwood in Australia recommends and describes a toluidine blue sulfation 
technique for rapid demonstration of Pneumocystis carinii.

This technique was rather widely recommended in the mid 1980's at the height 
of the AIDS epidemic - it was recommended in the standard Scott and Bailey 
microbiology textbook for instance - and then it sort of disappeared. Do many 
people have any actual experience of it - I've never seen it. I thought it 
was displaced by rapid GMS microwave techniques.

With concentrated sulfuric acid and acetone, it's a somewhat hazardous 
technique, particularly when performed by an inexperienced worker in the 
middle of the night ... and as for turning pathologists loose on it solo ...

Bob Richmond
Samurai Pathologist
Knoxville TN