Re: Paraffin processing brain slices

From:"John C. Dennis"


What kind of brain are you looking at?  How big are the slices you want to

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Wed, 23 Jan 2002, Bert Dotson wrote:

> A pharmacology researcher here assures me that a number of published
> studies indicate paraffin processing of 300 micron thick (thin if you ask
> me) slices of rat or mouse brain. Of course the procedure reported is
> "slices are paraffin processed and sectioned." Has anyone out there done
> this? Any particular tips or tricks? I am assuming a support medium like
> agar is used prior to processing.
> Thanx in advance
> Bert Dotson

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