Sarah
Onion root tip nuclei show well with Feulgen's technique or with Heidenhain's iron
hematoxylin. I am assuming that the dead areas will show negligible staining with either
of these techniques.
Barry


Sarah Ann Hunger wrote:

> Hello
>
> I am looking for a staining technique to differentiate between living and dead root
> tissue (preferably highlighting the dead tissue) in onion seedlings infected with
> Sclerotium cepivorum. I have dabbled with neutral red (Jensens stain), Phloroglucinol,
> and Fluoroscien diactetate without great success. Does anybody have any other stains
> and staining techniques (or improvements on the techniques I am currrently trying)
> they can suggest? The end purpose of this staining procedure is to improve the
> resolution between living and dead tissue for digital image analysis. Thank you in
> advance for your help.
>
> Kind regards
>
> Sarah
>
> Sarah A. Hunger M.Sc.
> Soil, Plant and Ecological Sciences Division
> PO Box 84
> Lincoln University
> Canterbury
> Phone: +64 3 325 2811 x 8157
> Mobile: +64 21 899 420
> Email: hungers@lincoln.ac.nz