Re: Mouse tissue processing protocol

From:Bryan Llewellyn

RE: Mouse tissue processing protocol
I too am of the opinion that overdehydration and overprocessing are myths.  Usually this artifact arises with small biopsies (often gut) which have been given minimal fixation in NBF, followed by rapid processing in a modern processor with full heat in the dehydration step.  I believe that the effects beeing seen are due to the tissue getting insufficient (or even no) chemical alteration from the formalin because the time is too short.  This is then followed by lousy fiixation in hot ethanol under the guise of dehydration.  Check out the old texts to see what ethanol fixation results in, then exaggerate it, and you will get a description of this artifact.  Perhaps the resolution is to develop a rapid fixative just for this purpose, but which protects the tissue against ethanol fixation.
Bryan Llewellyn
----- Original Message -----
From: Hewlett Bryan
To: ; 'Renee Hoyle-Thacker'
Sent: Thursday, January 17, 2002 5:36 AM
Subject: RE: Mouse tissue processing protocol

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