Re: Immunos on Mouse tissue

Hello Donella,

which organs do you try to stain ? How and how long are they fixed ?
We do a lot of stainings on mouse tissue with both polyclonal and
monoclonal Ab's (Dako ARK for the monoclonals). Since the tissue is so tiny
it might be overfixation can be a major problem.
Collagen might be difficult. We  invested a lot of time and energy to find
the right pretreatment to get it work on monkey tissue.
Hopefully some other histonetters out there can give you more

Antje Marcantonio
Novartis Pharma AG, Basle/Switzerland

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