In my experience, freezing the tissue prior to histological processing can reduce or even eliminate immunoreactivity for ER and PR. We did a small study years ago where we compared the immunoreactivity for ER and PR on the tissue used for the frozen section diagnosis and tissue that was not frozen. We saw several "false negatives". We always recommend using a "non-frozen" block of tissue for IHC, but, as you already know, this is not always possible. Please note that freezing can also affect S-100 immunoreactivity; this can result in a "false negative" when examining sentinel lymph nodes for metastatic malignant melanoma.
>>> anita dudley 01/23/02 11:42PM >>>
does anyone have any experience staining tissue for er-pr that has been
previously frozen? we had a small bx that was frozen in the or then
processed for permanent sections. the paths thought it should be positive
but they were both negative. does anyone have any data on this? thanks so
much, everyone have a nice day.
anita dudley providence hosp. mobile alabama
Join the world's largest e-mail service with MSN Hotmail.
<< Previous Message | Next Message >>