Re: Eosinophils and Mast cells.

From:Gayle Callis

Are you looking for mucosal mast cells or connective tissue mast cells?  If
you want to distinguish the two, you will need to do different staining
methods with different fixation methods.  

My favorite connective tissue mast cell stain is the Churukian Shenk
toluidine blue method from J Histotechnology back in early 80's. It gave no
background but wonderful, crisp staining for CTMC.  For mucosal, I did
Carnoys fixation, then an alcian blue (Enerbeck et al).   NBF fixed
tissues, with alcian blue for MMC was terrible.  

At 03:17 PM 1/18/02 +0000, you wrote:
>         Starting a small pilot project looking at eosinophil and mast cell
>distribution, so in the first instance I'll restrict myself to conventional
>techniques. Carbol chromotrope (Lendrum 1944) for the eosinophils, simple
>and very reliable. Mast cells, well, mmmm, so many choices but probably
>Methylene blue (Unna), Toluidine blue or Thionin (Cook 1961).
>            
>    
>  Dr. Ian Montgomery,
> Microscopy Service Unit,
> Graham Kerr Building,
>& Life Sciences,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 332 8855 Extn.6644.
> e-mail: ian.montgomery@bio.gla.ac.uk 
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)





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