Thanks for this info!  *g*

Connie M.

At 12:45 PM 1/24/02 -0500, Smith, Allen wrote:
>   Aldrich (1-800-558-9160) has cedarwood oil (#31,808-6), $43.10 plus 
>shipping for 500 ml. Carolina (1-800-334-5551) also has cedarwood oil
>(#8000-27-9), $23.65 plus  shipping for 100 ml.    Allen A. Smith    Barry
>University    Miami Shores, Florida    -----Original Message----- 
>From: Dr. Ian    Montgomery 
>Sent: Thu 1/24/2002 6:47 AM 
>To:    histonet@pathology.swmed.edu 
>Cc: 
>Subject: Fwd: Re:    "Overprocessed    tissue."
>
>Connie,
>          suitable schedules. John's book is    one of these texts everyone
>should have, a mine of solid gold nuggets.    Cedarwood oil is a bit
>expensive so you'd have to weigh the benefits before    using heavily. I
>routinely use chloroform overnight for clearing with a final    15 minutes
>in xylene as the link agent with wax. If it's a tissue I know will    be
>difficult then it gets cedarwood oil as the clearing agent.
>Ian.
>
>   X-Sender:      conmac@cc.usu.edu
>X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6      (32)
>Date: Wed, 23 Jan 2002 15:53:01 -0700
><>
><>
>Subject: Re: "Overprocessed      tissue."
>
>Ian,
>  Do you
>  I have only      heard of using this, never actually
>  I would love to      try this... if it is economically
>   Would you care to share your
>       
>
>much thanks from across the Pond
>Connie McManus in Utah      
>
>At 01:40 PM 1/23/02 +0000, you      wrote:
>>>     Recently there has been series of      postings regarding
overprocessed
>tissue that have left me totally      confused. The object in processing and
>embedding in paraffin wax is the      complete removal of water from
tissue and
>  tissue
>has been lost and      replaced by wax. If that is what's meant then the
>overprocessed tissue is      only fit for the rubbish bin. I really think
that
>the term overprocessing      should be dropped and replaced by something more
>suitable and probably      more accurate such as under processed,
>mis-processed,
>it wasn't me it was      the machine and so forth. From the descriptions
people
>give I think water      is still present in the tissue or users have moved
away
>from "proper"      clearing agents and rely on xylene or a substitute as the
>agent of choice      which can result in "crumbly" blocks. I still use
>chloroform, cedarwood      oil etc., "real" clearing agents and when the
tissue
>has been thoroughly      dehydrated, cleared and embedded the resulting
blocks
>are a dream.      
>>        Then we have the next wee      problem. "How long did you fix the
>tissue." "Oh, not very long, it's only      been in Bouin for a couple of
>months. That's alright, isn't      it."
>>Ian.
>>
>
>Veterinary Diagnostics Lab
>Utah State      University
>Logan, UT
>USA
>fax (435) 797-2805
>   Dr. Ian Montgomery,
>Microscopy Service    Unit,
>Graham Kerr Building,
>& Life    Sciences,
>University of Glasgow,
>Glasgow,
>G12 8QQ.
>Tel: 0141 332    8855 Extn.6644.
>e-mail: ian.montgomery@bio.gla.ac.uk  
> 

Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA
fax (435) 797-2805