Biogenex provided a wall chart with antibodies and what retrievals they
recommended. Don't know if that is still available, but I am not sure if
HIER was included.  

At 08:45 PM 1/25/02 -0500, you wrote:
>For commercially supplied / prediluted antibodies, for either manual or 
>automatic staining, suppliers have HIER pretreatment (scoring) tables that 
>are very helpful to use as guidelines on which antibodies need or not need 
>HIER and what kind of retireval solution to use. 
>
>Rolly Perez BS, HT(ASCP)
>Portland, Oregon
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)