Re: Antigen retrieval

From:rueggp

The point of using Zinc formalin is that it will in most cases illiminate the need for antigen retrieval because cross-linking has not occured to the extent it will with formalin.  If you are going to use HIER anyway, why bother to switch to zinc formalin.  You could run some comparison tissues fixed in formalin with hier, fixed in zinc formalin with and without hier.
Patsy

Laurie Colbert wrote:

I was wondering if it was necessary to use zinc formalin to enhance IHC staining and prevent cross-linking if you were doing antigen retrieval for every antibody.  Any comments???

Laurie Colbert


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