I have always used saturated aqueous lithium carbonate for blueing
hematoxylin.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695 

-----Original Message-----
From: Cohen, Mary Ann [mailto:mcohen@bioreliance.com]
Sent: Thursday, January 31, 2002 8:46 AM
To: 'histonet@pathology.swmed.edu'
Subject: lithium carbonate



  Hi all,

My question is,  for an H&E stain when making up your lithium carbonate
solution after your Hematox dip, I am using a saturated aqueous lithium
carbonate solution with 1 gram of lithium into 100 ml of  ( this is the
question ) do I use distilled H2O, or 100 % EtOH?  If my memory serves me
after many years of working in the lab and smelling all of the chemicals,  I
would use EtOH,   is this correct.

I would appreciate your comments.

Food for thought,  I do not want to use pre-made clarifier.

Thanks,

MaryAnn Cohen
Pathology Assistant
MTS II
BioReliance
9630 Medical Center Drive
Rockville, MD 20850-3349
301-610-2192
mcohen@bioreliance.com  
fax # 301-838-0371