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Vinnie,
 
I have not used recycled formalin, though in theory it should work as well as the unrecycled product.  I'd say the best way to test the impact is to do side-by-side comparison with tissue using the recycled vs unrecycled product.  Process them together in the same run and then do the immunostains and see what you get.  Let us know how it turns out.


Teri Johnson 
Manager Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, Missouri  64110 
tjj@stowers-institute.org 

 
 -----Original Message-----
From: Vinnie Della Speranza [mailto:dellav@musc.edu]
Sent: Tuesday, January 15, 2002 4:17 PM
To: histonet@pathology.swmed.edu
Cc: John Metcalf; Mary Richardson
Subject: immunohistochemical staining on tissues fixed with recycled formalin



Has anyone seen aberrant immunohistochemical staining that might be attributable to fixing tissues with recycled formalin ? 
We have been using a formalin recycler on demo. each run has been assayed for concentration, adjusted if necessary and re-buffered.  The recycled product appears morphologically to be performing as expected but the question of impact on immuno-staining has been raised.
 
thank you for your views on this.
 


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Vinnie,
 
I have not used recycled formalin, though in 
theory it should work as well as the unrecycled product.  I'd say the best 
way to test the impact is to do side-by-side comparison with tissue using the 
recycled vs unrecycled product.  Process them together in the same run and 
then do the immunostains and see what you get.  Let us know how it turns 
out.



Teri Johnson 
Manager Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, 
Missouri  64110 
tjj@stowers-institute.org 
 
 -----Original 
Message-----
From: Vinnie Della Speranza 
[mailto:dellav@musc.edu]
Sent: Tuesday, January 15, 2002 4:17 
PM
To: histonet@pathology.swmed.edu
Cc: John Metcalf; Mary 
Richardson
Subject: immunohistochemical staining on tissues fixed with 
recycled formalin


Has anyone seen aberrant 
  immunohistochemical staining that might be attributable to fixing tissues 
  with recycled formalin ? 
  
We have been using a formalin recycler on demo. each run has been assayed 
  for concentration, adjusted if necessary and re-buffered.  The recycled 
  product appears morphologically to be performing as expected but the question 
  of impact on immuno-staining has been raised.
  
 
  
thank you for your views on this.
  
 

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