RE: Skin problems

From:Van Hecke Donald

Hi,

Have you controlled your cutting temperature ? To hot or to cold can make
the cutting more difficult. OriŽntation can make the cutting easier, as you
may know. I advise you to collect your sections on Silanocoated slides, to
minimise the lost, even with micrwave treatment.
Lots of succes,
Donald Van Hecke
AZ St. Lucas - Brugge
Belgium.

> -----Oorspronkelijk bericht-----
> Van:	Kavita Joory [SMTP:Kavita.Joory@bristol.ac.uk]
> Verzonden:	vrijdag 25 januari 2002 12:59
> Aan:	histonet@pathology.swmed.edu
> Onderwerp:	Skin problems
> 
> Can anyone suggest ways to make paraffin embedded human skin easier to
> cut ? we have tried mollifex but still have problems.  Also our skin
> sections tend to come off in the microwave (we are using gelatin coated
> slides) any help would be greatly appreciated
> 
> kind regards
> 
> Kavita Joory
> 
> --
> Kavita Joory
> Research Assistant
> Department of Physiology
> University of Bristol
> School of Pre-clinical Veterinary Sciences
> Southwell Street
> BRISTOL
> United Kingdom
> BS2 8EJ
> 
> Telephone: 0117 928 7416
> 
> 




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